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Establishment of a xeno-free culturing system for <t>ESC</t> lines and <t>iPSC</t> lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.
Psgro® Human Ipsc/Esc Growth Medium, supplied by StemRD Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psgro-free human ipsc/esc growth medium  (StemRD Inc)
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Establishment of a xeno-free culturing system for <t>ESC</t> lines and <t>iPSC</t> lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.
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Establishment of a xeno-free culturing system for <t>ESC</t> lines and <t>iPSC</t> lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.
Psgro ® Human Ipsc/Esc Growth Medium, supplied by StemRD Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/psgro ® human ipsc/esc growth medium/product/StemRD Inc
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Establishment of a xeno-free culturing system for <t>ESC</t> lines and <t>iPSC</t> lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.
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Establishment of a xeno-free culturing system for ESC lines and iPSC lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.

Journal: Protein & Cell

Article Title: Long-term in vivo chimeric cells tracking in non-human primate

doi: 10.1093/procel/pwad049

Figure Lengend Snippet: Establishment of a xeno-free culturing system for ESC lines and iPSC lines from monkey preimplantation embryos and fibroblasts. (A) Morphology of monkey ESCs colonies before and after conversion using the XF-PSC medium (upper) (a, cultured in a primed medium; b, conversion of cells; c, cultured in XF-PSC medium). Establishment of monkey iPSCs and ESCs from fibroblast (middle) (a, fibroblast reprogramming; b, iPSC clone; c, iPSCs cultured in XF-PSC medium) and monkey day 6 embryos (lower) (a, outgrowth; b, isolated single cell; c, ESCs cultured in XF-PSC medium). Scale bar, 100 μm. (B) Analysis of doubling time of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P = 0.0044. (C) Single-cell cloning efficiency of primed ESCs and xeno-free ESCs. n = 3 biologically independent samples, data are mean with SD, unpaired two-tailed t -test, P < 0.0001. (D) Immunostaining of pluripotency marker genes expression in establishment monkey iPSCs (upper). Immunostaining of pluripotency marker genes expression in establishment monkey ESCs (lower). Scale bar, 100 μm.

Article Snippet: Conventional primed monkey PSCs were cultured in DMEM/F12 (Thermo Fisher Scientific) with 15% KSR (Gibco) containing 10 ng/mL bFGF (Peprotech), 0.1 mmol/L β-Me (STEMCELL), NEAA (Thermo Fisher Scientific), and 20% PSGro® Human iPSC/ESC Growth Medium (StemRD).

Techniques: Cell Culture, Isolation, Two Tailed Test, Clone Assay, Immunostaining, Marker, Expressing